1. Mix: Combine ingredients in a 2L flask. Drop in magnetic stir bar.
2. Dissolve: Stir on hot plate (low/no heat) until perfectly dissolved.
3. Sterilize: Foil cover. Autoclave at 121°C (15 PSI) for 45 mins.
4. Inoculate: Room temp, move to Flow Hood. Inject 10ml purchased LC OR drop 2 Master Agar wedges. Incubate on stirrer.