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Atish's Mushroom Lab

Swabhimaan Bio • 1000 Jar / Cycle

AC + HEPA: 20°C - 22°C Humidity: 50% Autoclave: 14 Jars

Total Progress

Yields enough for 5 Autoclave runs (70 Jars). 70 x 70ml = 4.9L. 5L prevents wastage while allowing a 100ml buffer.

50g Brown Rice

70ml Daily Broth

6-8ml Liq. Culture

1. Mix: Combine ingredients in a 1L Erlenmeyer flask. Drop in magnetic stir bar.

2. Heat: Place on hot plate stirrer. Heat and stir until clear (no clumps).

3. Sterilize: Cap with foil. Autoclave at 121°C (15 PSI) for 45 mins.

4. Pour: Let cool to ~45°C. In front of HEPA Hood, pour into sterile petri dishes.

1. Mix: Combine ingredients in a 2L flask. Drop in magnetic stir bar.

2. Dissolve: Stir on hot plate (low/no heat) until perfectly dissolved.

3. Sterilize: Foil cover. Autoclave at 121°C (15 PSI) for 45 mins.

4. Inoculate: Room temp, move to Flow Hood. Inject 10ml purchased LC OR drop 2 Master Agar wedges. Incubate on stirrer.